Microbial Community Analysis of Secondary Composting Fermentation of Porcine Fermented Mattress Materials
porcine fermented mattress materials were used as the research objects,and humic acid,calcium superphosphate and ferrous sulfate were used as nitrogen retention agents for composting fermentation.The composition of microbial community in the fermentation products was analyzed,and the effects of different nitrogen retention agents were evaluated from the perspective of composting beneficial bacteria,so as to provide theoretical basis for further composting of porcine mattress materials.High-throughput sequencing technology was used to analyze the structural differences of bacteria and fungi in fermentation products treated with different nitrogen preserving agents at the phylum and genus levels.The results showed that at the phylum level,Chloroflexi,Proteobacteria,Actinobacteriota,Bacteroidota,and Firmicutes were the dominant phyla in the fermentation products.At bacteria genera level,removing the unnamed and unclassified bacteria,the main bacterium in the fermented product were Bacillus,Streptomyces and Pseudomonas.The application of humic acid increased the abundance of Proteobacteria,Bacteroidetes and Pseudomonas,while decreased the richness of Chloroflexi,Actinobacteria,Bacillus and Streptomyces.Calcium superphosphate enhanced the abundance of Chloroflexi,but reduced that of Proteobacteria,Firmicutes,Bacillus and Pseudomonas.Ferrous sulfate increased the abundance of Bacteroidetes and Streptomyces,but decreased that of Chloroflexi,Firmicutes,Bacillus and Pseudomonas.At the fungi phylum level,Ascomycota was the dominant group in the fermentation products.At the fungi genus level,the treatments with calcium superphosphate and ferrous sulfate resulted in the prevalence of Trichoderma and Aspergillus.Humic acid treatment significantly reduced the abundance of Trichoderma and As-pergillus,leading to a more even distribution of fungi richness,with a notable increase in the abundance of beneficial fungi such as Pseudallescheria and Candida,which are conducive to fermentation.Therefore,the addition of different nitrogen conservation agents during the secondary fermentation of composting bed substrates can alter the diversity and richness of bacteria and fungi communities in the fermentation products.Among them,humic acid exhibits the most significant effect on community richness,with a lower inhibitory effect on beneficial bacteria compared to calcium superphosphate,and its impact on richness is greater than that of ferrous sulfate.
万方数据
维普
