Effects of Dietary Berberine on Growth Performance,Antioxidant Capacity,Lipid Metabolism and Intestinal Health in Zebrafish Fed High-fat Diet
Five diets with 5%fat diet(CK),11%fat diet(high-fat group),and three 11%fat diets supplemented with 100,200,and 300 mg/kg of berberine were prepared and recorded as LL,HL,HL100,HL200 and HL300 groups,respectively.A total of 450 zebrafish were randomly divided into 5 treatment groups with 3 replicates per group and 30 fish per replicate for 8 weeks.The results showed that there were no significant differences in growth performance among all groups,but the high-fat diet(HL)had significant negative effects on liver and intestinal health.Compared with the HL group,the contents of T-CHO,TG and LDL-C and the activities of SOD,CAT,GSH,GPX,HL,LPL and TL in liver of zebrafish in the HL300 group were significantly increased,while the contents of MDA and HDL-C in liver were significantly decreased.Supplementation of 200～300 mg/kg berberine in a high-fat diet can effectively reduce the enrichment of liver fat and increase the height of intestinal villi.In addition,dietary berberine significantly decreased the relative abundance of Actinobacteria and Mycobacterium in the intestines of zebrafish fed high-fat diets,while supplementation with 300 mg/kg berberine significantly increased the relative abundance of Bacteroides,and significantly decreased the relative abundance of Rolstonia.In conclusion,dietary berberine had no significant growth-promoting effect on zebrafish fed with high-fat diets,but supplementation of 300 mg/kg berberine in high-fat diets could significantly improve liver antioxidant capacity,liver lipid metabolism and intestinal health of zebrafish.
万方数据
维普
