UPLC-MS/MS Method for Determination of Multiple Drug Residues in Pork
The samples were extracted with 80%acetonitrile solution,purified with PRiME HLB solid phase extraction column,concentrated with nitrogen,and finally dissolved with 10%methanol.A gradient elution was performed using 0.01%formic acid water-methanol as a mobile phase for gradient elution.The multi-reaction monitoring(MRM)mode of UPLC-MS/MS was used for the determination,and quantification was performed by internal standard method.The results showed that the concentration of 30 compounds showed good linearity in the range of 0.5～20.0 ng/mL,with the coefficient correlation(R2)between 0.993997 to 0.999872.At the addition levels of 1.0,5.0 and 10.0 μg/kg,the recovery ratio of 30 compounds was 85.9%～115.8%in pork.The detection limit of 30 compounds was 0.02～1.00 μg/kg,and quantification limit was 0.06～3.00 μg/kg.The test indicated that the method was simple,sensitive,accurate,stable in recovery,good accuracy and repeatability,and suitable for the detection and analysis of large quantities of pork samples.
UPLC-MS/MSPorkDrugResiduesGradient elutionInternal standard method
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