基于微流控技术的单细胞电学特性研究
Electrical Characteristics of Single Cells with Microfluidic
郑磊 1黄明宇 1张华丽1
作者信息
- 1. 南通大学机械工程学院,江苏 南通 226019
- 折叠
摘要
将微流控技术与库尔特计数器相结合,基于细胞电学特性对细胞进行无标记检测.该检测技术在细胞定量分析及分选中具有很大的优势.这里采用数值仿真的方法研究了成熟红细胞在直流和交流电源下的电学特性,仿真结果表明,不同细胞大小在电极检测区域引起的电信号变化,显示出不同的电学特性.为验证仿真结果,基于微流控技术对浓度为105个/ml的聚苯乙烯微球,在3V直流电源下所引起的电流变化进行研究.研究结果表明,该模型可以较好的反应细胞在电极检测区域内的电学特性.
Abstract
The microfluidic technology is combined with the Coulter counter to perform label-free detection of cells based on the electrical characteristics of cells.This detection technology has great advantages in cell quantitative analysis and sorting.It uses numerical simulation to study the electrical characteristics of mature red blood cells under DC and AC power supplies.The simula-tion results show that the electrical signal changes caused by different cell sizes in the electrode detection area show different elec-trical characteristics.In order to verify the simulation results,based on the microfluidic technology,the current change caused by the polystyrene microspheres with a concentration of 105/ml under a 3V DC power supply was studied.The research results show that the model can better reflect the electrical characteristics of cells in the electrode detection area.
关键词
微流控技术/库尔特计数器/单细胞模型/电学特性/数值仿真Key words
Microfluidic/Coulter Counter/Single Cell Model/Electrical Characteristics/Numerical Simulation引用本文复制引用
基金项目
江苏省自然科学基金资助项目(BK20150406)
江苏省高校优势学科建设工程资助项目(苏财政2018-192号)
出版年
2023
NETL
NSTL
维普
万方数据