首页|基于微流控技术的单细胞电学特性研究

基于微流控技术的单细胞电学特性研究

扫码查看
将微流控技术与库尔特计数器相结合,基于细胞电学特性对细胞进行无标记检测.该检测技术在细胞定量分析及分选中具有很大的优势.这里采用数值仿真的方法研究了成熟红细胞在直流和交流电源下的电学特性,仿真结果表明,不同细胞大小在电极检测区域引起的电信号变化,显示出不同的电学特性.为验证仿真结果,基于微流控技术对浓度为105个/ml的聚苯乙烯微球,在3V直流电源下所引起的电流变化进行研究.研究结果表明,该模型可以较好的反应细胞在电极检测区域内的电学特性.
Electrical Characteristics of Single Cells with Microfluidic
The microfluidic technology is combined with the Coulter counter to perform label-free detection of cells based on the electrical characteristics of cells.This detection technology has great advantages in cell quantitative analysis and sorting.It uses numerical simulation to study the electrical characteristics of mature red blood cells under DC and AC power supplies.The simula-tion results show that the electrical signal changes caused by different cell sizes in the electrode detection area show different elec-trical characteristics.In order to verify the simulation results,based on the microfluidic technology,the current change caused by the polystyrene microspheres with a concentration of 105/ml under a 3V DC power supply was studied.The research results show that the model can better reflect the electrical characteristics of cells in the electrode detection area.

MicrofluidicCoulter CounterSingle Cell ModelElectrical CharacteristicsNumerical Simulation

郑磊、黄明宇、张华丽

展开 >

南通大学机械工程学院,江苏 南通 226019

微流控技术 库尔特计数器 单细胞模型 电学特性 数值仿真

江苏省自然科学基金资助项目江苏省高校优势学科建设工程资助项目

BK20150406苏财政2018-192号

2023

机械设计与制造
辽宁省机械研究院

机械设计与制造

CSTPCD北大核心
影响因子:0.511
ISSN:1001-3997
年,卷(期):2023.394(12)
  • 4