首页|传染病教学中开设多色流式细胞术课程的实践

传染病教学中开设多色流式细胞术课程的实践

Practice of Multicolor Flow Cytometry in the Teaching of Infectious Diseases

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多色流式细胞术是一种高效、精准、快速的细胞学检测技术,已广泛应用于传染病检测和研究中.在传染病研究生教学中,本团队建立多色流式课程体系并通过教学实践过程总结教学经验.文章阐述了传染病研究生教学中开设多色流式细胞术课程的几个方面,并进行了总结,包括确定课程目标、设计课程结构、确定课程内容、确定课程形式和确定课程大纲五个方面.笔者进一步通过重点推动多色流式细胞术实践课程并总结实践的初步经验,包括明确多色流式细胞术可以检测的样本类型、多色流式细胞术的仪器类型、染色策略和实验方案、加强流式数据分析和结果 判读能力、多色流式细胞术实践过程中应强化生物安全操作五个要点.该课程的开展有助于传染病研究生对免疫学和传染病发病机制的理解,为其未来从事传染病防治和免疫学研究的教学工作提供坚实的基础.
Multicolor flow cytometry is a widely used cytological detection technique in the field of infectious diseases.In order to enhance the teaching of infectious disease graduate students,the team has developed a comprehensive multi-color flow course system and gained valuable teaching experience through practical application.This paper focuses on several key aspects of the multi-color flow cytometry course for infectious disease graduate students,including determining the course objectives,designing the course structure,selecting the course content,deciding the course format,and outlining the course.Additionally,the author emphasizes the importance of the practical application of multicolor flow cytometry and shares preliminary experiences,such as identifying the sample types that can be detected using this technique,understanding the different instrument types,discussing staining strategies and experimental schemes,enhancing the ability to analyze stream data and interpret results,and emphasizing biosafety protocols during multicolor flow cytometry practices.The development of this course will greatly contribute to the students'understanding of immunology and the pathogenesis of infectious diseases,and will provide them with a strong foundation for their future work in infectious disease prevention and immunology research.

multicolor flow cytometryinfectious diseasesgraduate studenteducationcurriculum provisionteaching practice

杜娟、赵红心、张瓃丹、王芯栎、孔雅娴

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首都医科大学附属北京地坛医院传染病研究所/新发突发传染病研究北京市重点实验室,北京 100015

北京市感染性疾病研究中心,北京 100015

国家传染病医学中心/首都医科大学附属北京地坛医院研究所,北京 100015

国家传染病医学中心/首都医科大学附属北京地坛医院艾滋病临床中心,北京 100015

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多色流式细胞术 传染病 研究生 教学 课程设置 教学实践

北京市自然科学基金北京市医院管理中心"登峰"人才培养计划项目高层次公共卫生技术人才建设项目北京市医院管理中心重点医学专业发展计划建设项目

M21007DFL20191802学科骨干-02-27ZYLX202126

2024

10.3969/j.issn.1004-6763.2024.01.035

继续医学教育
天津医学高等专科学校

继续医学教育

影响因子：0.637
ISSN：1004-6763
年,卷(期)：2024.38(1)
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