大蒜抗菌活性蛋白的分离、纯化和鉴定
Isolation,Purification and Identification of Antimicrobial Proteins from Allium Sativum
魏雪盈 1徐栋梁 2闫浩 2夏立新 2刘志刚2
作者信息
- 1. 深圳大学医学院临床医学专业2011级,广东,深圳,518060
- 2. 深圳大学医学院过敏反应与免疫学研究所,广东,深圳,518060
- 折叠
摘要
目的 从大蒜中分离纯化具有抗真菌活性的蛋白.方法 提取大蒜粗提液,通过DEAE-SephadexTM A-50阴离子交换层析、Affi-gel blue gel亲和层析和ResourceTM S阳离子交换层析纯化目的 蛋白.通过Tricine-SDS-PAGE、抗真菌活性检测鉴定该蛋白对不同真菌的抑制活性.结果 纯化得到的蛋白分子质量为13 ku,该蛋白对落花生褐斑病菌有明显的生长抑制活性,而对绿色木霉、黑曲霉、白地霉和产黄青霉没有生长抑制活性.纯化得到另外一种蛋白的分子质量约为4.6 ku,该蛋白对绿色木霉有明显的生长抑制活性,而对落花生褐斑病菌、黑曲霉、白地霉和产黄青霉没有生长抑制活性.结论 大蒜中存在抗真菌活性蛋白.
Abstract
Objective To isolate and purify the protein with antif ungal activity from Allium sativum. Methods Crude extracts were obtained from Allium sativum and antimicrobial proteins were purified by ion exchange chromatography on DEAE-SephadexTM A-50, affinity chromatogra-phy on Affi-gel blue gel, and ion exchange chromatography on ResourceTM S. The antif ungal activity of the purified proteins was identified by Tricine-SDS-PAGE and antifungal activity test. Results The molecular mass of the purified protein was 13 ku. The protein inhibited the growth of Mycosphaerella arachidicola , but had no effects on Trichoderma viride , Aspergillus niger , Geotrichum candidum and Penicillium chrysogenum. In addition, another purified protein had a molecular mass of 4. 6 ku. The protein inhibited the growth of Trichoderma viride , but had no effects on Mycosphaerella arachidicola , Aspergillus niger , Geotrichum candidum and Penicillium chrysogenum. Conclusion Antimicrobial proteins isolated from Allium sativum can prohibit the growth of some fungi.
关键词
大蒜/离子交换层析/亲和层析/活性抑制Key words
Allium sativum/ion-exchange column chromatography/affinity chromatography/activity prohibition引用本文复制引用
基金项目
国家自然科学基金(81271950)
深圳市重点实验室组建项目(SW201110010)
出版年
2012
NETL
NSTL
维普
万方数据