Objective To investigate the effect of tiotropium bromide on asthma through the MAPK/NF-κB signaling pathway and its potential mechanism.Methods Airway smooth muscle cells(ASMCs)were treated with 10 ng·mL-1 platelet-derived growth factor-BB(PDGF-BB)and different concentrations oftiotropium bromide(0,5,10,20 and 50 μmol·L-1).The prolifera-tion,colony formation ability,migration and invasion of ASMCs were analyzed by CCK-8,colony-formation,scratch wound healing and Transwell assays,respectively.The expression levels of MMP2,MMP9,calponin,α-SMAand MAPK/NF-κB signaling pathway proteins were analyzed by Western blot.Results Tiotropium bromide inhibited PDGF-BB-induced proliferation,migration and invasion and modulated phenotypic transformation in ASMCs(P＜0.05).Furthermore,tiotro-pium bromide inhibited DGF-BB-caused activation of MAPK/NF-κB signaling pathway,and re-duced the expression of p-38,p-JNK,p-ERK,p-p65 and p-NF-κB(P＜0.05).Conclusion Tiotro-pium bromide can inhibit the proliferation and migration of ASMCs by blocking MAPK/NF-κB signal transduction.It is speculated that tiotropium bromide can be used as a promising drug for the treatment of asthma.
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