Construction and Functional Characterization of Rat VDAC1 Adenovirus Overexpression Vector
Objective In order to lay a foundation for exploring the mechanism of action of voltage-dependent anionic channel protein 1(VDAC1)in myocardial ischemia-reperfusion injury,an adenovirus vector overexpressing VDAC1 was constructed and its expression in H9 C2 cells was observed.Methods ADV6-NC adenovirus and ADV6-VDAC1 adenovirus were constructed,and H9c2 myocardial cells of the 6th generation were used for transfection.The cells were divided into three groups:blank control group,negative control group(transfection with ADV6-NC)and experimental group(transfection with ADV6-VDAC1).The expression of VDAC1 was detected by qPCR and Western blot.Results The ADV6-NC and ADV6-VDAC1 vectors were constructed successfully.The expression of VDAC1 in the experimental group was obviously higher than that in the blank control group and the negative control group(P＜0.05).Conclusion H9c2 cells can express VDAC1 constantly and efficiently by using adenovirus vector in vitro.
voltage-dependent anionic channel protein 1(VDAC1)myocardial cellsplasmid constructionadenovirus vector
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