Naringin Alleviates Adipocyte Inflammation after Palmitic Acid Treatment through Inhibiting ERK1/2 Activation and DRP1 Expression
Objective To investigate the effects and mechanism of narinin(Nar)on inflamma-tory response of mature adipocytes after palmitic acid(PA)treatment.Methods After 3 T3-L1 pre-adipocytes were induced into mature adipocytes by lipogenesis,the cells were treated with dif-ferent concentrations(0,25,50,100,200μmol·L-1)of PA for 48 h.Cell viability was detected by CCK-8,and the level of IL-6 in the culture supernatant was detected by ELISA to determine the optimal PA concentration for subsequent experiments.Mature adipocytes were treated with differ-ent concentrations(0,12.5,25,50,100 μmol·L-1)of Nar,and cell viability was detected by CCK-8 to determine the optimal Nar concentration for subsequent experiments.PA treatment experi-ments were divided into 2 groups:Ctrl group and PA group;Nar treatment experiments were di-vided into 4 groups:Ctrl group,PA group,Nar group,and PA+Nar group;western blotting was used to detect the protein expression levels of p-ERK1/2 and DRP1 in each group.Mitochondrial fission inhibitor Mdivi-1 treatment experiments were divided into 7 groups:Ctrl group,PA group,Nar group,Mdivi-1 group,PA+Nar group,PA+Mdivi-1 group,and PA+Nar+Mdivi-1 group;ELISA and western blotting were used to detect the levels of IL-6 in the supernatant of the 7 groups,and the levels of p-ERK1/2 and DRP1 protein expression,respectively.Results In follow-up experiments PA and Nar were selected at 50 and 25 μmol·L-1,respectively.p-ERK1/2 and DRP1 expression levels were elevated in the PA group compared with the Ctrl group(P＜0.001).Compared with the PA group,IL-6 level,p-ERK1/2 and DRP1 expression were lower in the PA+Nar,PA+Mdivi-1,and PA+Nar+Mdivi-1 groups(P＜0.05);compared with the PA+Nar group,the difference in IL-6 level was not statistically significant in the PA+Nar+Mdivi-1 group(P＞0.05),but p-ERK1/2 and DRP1 expression levels were both reduced(P＜0.05).Conclusion The results suggest that Nar can alleviate the PA-induced adipocyte inflammation by inhibiting ERK1/2 activation and DRP1 expression.
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