Effects of Traditional Chinese Medicine Tongxiaoyin on Primary Dysmenorrhea with Syndrome of Congealing Cold with Blood Stasis and Its Effect on MAPK/ERK Signaling Pathway
Objective To investigate the effect of traditional Chinese medicine Tongxiaoyin on primary dysmenorrhea with syndrome of congealing cold with blood stasis and its effect on mito-gen-activated protein kinase/extracellular regulated protein kinase(MAPK/ERK)signaling path-way.Methods A total of 106 patients with primary dysmenorrhea due to cold coagulation and blood stasis syndrome were selected from Hengshui City Hospital of Traditional Chinese Medi-cine and divided into traditional Chinese medicine group and conventional group according to ran-domized numerical table method,each with 53 cases.The conventional group was treated with ibu-profen granules,while the traditional Chinese medicine group was treated with Tongxiaoyin.We compared the scores of Cox dysmenorrhea symptom scale(CMSS),pain visual analogue scales(VAS),serum pain mediator levels[β-Endorphin(β-EP),Prostaglandin F2α(PGF2α),Prostaglan-din E2(PGE2)],peripheral blood mononuclear cell MAPK/ERK signaling pathway related factor levels[mitogen-activated extracellular signal-regulated kinase(MEK)1,MEK2,ERK1,ERK2 mRNA],uterine microcirculation status[Uterine arterial blood flow pulsatility index(PI),resist-ance index(RI),systolic and diastolic blood flow velocity ratio(S/D)]before and after the treat-ment,and the incidence of adverse reactions between the two groups.Results The total effective rate of the traditional Chinese medicine group was higher than that of the conventional group(P＜0.05).The post-treatment VAS score,CMSS duration and severity score of the 2 groups were low-er than those before treatment,and they were lower in the traditional Chinese medicine group than in the conventional group(P＜0.05);the post-treatment serumβ-EP and PGE2 levels of the 2 groups were higher than those before treatment,and they were lower in the traditional Chinese medicine group than in the conventional group(P＜0.05);the post-treatment PGF2αlevels,the peripheral blood single nucleated cells MEK1,MEK2,ERK1,ERK2 mRNA relative expression,u-terine artery PI,RI,S/D were lower than those before treatment,and they were lower in the tra-ditional Chinese medicine group than in the conventional group(P＜0.05).The difference in the incidence rate of adverse reactions between the 2 groups was not statistically significant(P＞0.05).Conclusion Tongxiaoyin can have a significant therapeutic effect on primary dysmenorrhea due to syndrome of congealing cold with blood stasis.It can significantly alleviate dysmenorrhea symptoms by inhibiting the expression of factors related to the MAPK/ERK signaling pathway and pain mediators,and improving the uterine microcirculation status,with a good safety profile.
primary dysmenorrheasyndrome of congealing cold with blood stasisTongxi-aoyin,TCM drugmitogen-activated protein kinaseextracellular regulated pro-tein kinasesignaling pathway
万方数据
维普
