Organoids,as in vitro 3 D models which maintain their own tissue structure and function,have largely overcome the limitations of traditionally-cultured tumor models,including 2 D cell culture and in vivo mouse culture,and organoid culture has become a powerful tool in tumor research in recent years.Hepatocellular carcinoma(HCC)organoids are generated from tis-sue samples of human and mouse origin and retain key features of parental tumor tissues,inclu-ding histological structure,genomic landscape,expression profile,and intratumoral heterogeneity.The application of organoids in exploring the mechanism of hepatitis B virus(HBV)and other factor-induced hepatocellular carcinoma,drug screening,and individualized medical treatment is currently being widely investigated.This article provides a review on the origin and characteriza-tion of organoids and their application in hepatocellular carcinoma.
关键词
类器官/肝细胞癌/临床前研究模型/HBV感染
Key words
organoids/hepatocellular carcinoma/preclinical research model/HBV infection
维普
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