Emodin Inhibits Proliferation of Vemurafenib-resistant Melanoma Cells
Objective To investigate the in vitro inhibitory effect of emodin on vemurafenib-re-sistant melanoma cells(A375R).Methods A375R cells in Control Group and Group A-C were treated with 0,80,160,and 320 μmol·L-1 emodin for 48 h.The effects of emodin on cell prolifer-ation,apoptosis and the expression of key proteins(Bcl-2,Akt,p-Akt,mTOR,p-mTOR)of PI3K/AKT/mTOR signaling pathway were detected by CCK-8 assay,flow cytometry and western blot-ting in the groups,respectively.Results Compared with Control Group,the cell proliferation inhi-bition and apoptosis rates were significantly increased in a concentration-dependent manner in emodin-treated Group A-C(P＜0.01 and P＜0.05,respectively).The expression levels of Bcl-2 protein in the cells of Groups A-C were significantly reduced(P＜0.05),and the expression levels of p-Akt and p-mTOR proteins were decreased,of which the differences between Groups B and C and Control group were statistically significant(P＜0.05).Conclusion Emodin can inhibit prolif-eration and induce apoptosis in vemurafenib-resistant melanoma A375R cell by a mechanism that may be related to the inhibition of PI3K/AKT/mTOR signaling pathway.
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