Regulation of Autophagy and mTOR Signaling Pathway Genes in Respiratory Epithelial Regulation of Autophagy and mTOR Signaling Pathway Genes in Respiratory Epithelial Cells of Children with Upper Respiratory Tract Infection by Respiratory Syncytial Virus
Objective To investigate the effects of respiratory syncytial virus(RSV)on autoph-agy and mTOR signaling pathway-related genes in respiratory epithelial cells of children with up-per respiratory tract infection.Methods A total of 145 children with upper respiratory tract infec-tion admitted to Tangshan Vocational and Technical College Affiliated Hospital from November 2022 to December 2023 were selected as the study objects.At the same time,age-and sex-matched healthy children(n=10)who underwent physical examination in our hospital during the same pe-riod were included as the healthy control group.Nasopharyngeal aspirate(NPA)were collected from all children.RSV was screened by RT-PCR,and the molecular characteristics of RSV strains were determined by G(attachment)gene amplification and phylogenetic analysis was performed.The expression levels of respiratory epithelial cell autophagy-related genes(BECN1,ATG3,ATG5,NPC1 and GABARAP)and mTOR signaling pathway-related genes(AKT1,mTOR,PD-PK1,RICTOR,and TSC1)were detected using qRT-PCR in 25 RSV-positive NPA samples(RSV Group)and 10 healthy control NPA samples(Healthy Control Group).Results Of 145 NPA samples from children with upper respiratory tract infection,69(47.6％)were RSV positive.The circulating genotypes of 31 representative RSV strains were detected and BLAST analysis showed that all 31 RSV strains were RSV-A type.By targeting the ectodomain portion of the G gene,phy-logenetic analysis showed that 31 strains of RSV-A were clustered into ON1 genotypes with 72 bp nucleotide repeats.In addition,the strains belonged to lineage 1(51.6％),followed by lineage 3(29％)and lineage 2(19.4％).The mRNA expression levels of ATG3 and NPC1 in respiratory ep-ithelial cells of the RSV group were significantly higher than those of the healthy control group(P＜0.001).The mRNA expression levels of AKT1,mTOR and TSC1 in respiratory epithelial cells of the RSV group were significantly lower than those of the healthy control group(P＜0.001).Conclusion RSV infection can increase the expression of autophagy-related genes(ATG3 and NPC1)in respiratory epithelial cells of children with upper respiratory tract infection,inhibit the expression of mTOR signaling pathway-related genes(AKT1,mTOR and TSC1),and then e-vade the host immune system to achieve their survival mode in the host.
NETL
NSTL
万方数据
