Resveratrol Inhibits Oral Squamous Cell Carcinoma Cells Proliferation,Migration and Glycolysis
Objective To investigate the effects of resveratrol(RES)on the proliferation,migration,cell cycle and glycolysis of oral squamous cell carcinoma(OSCC)cells and its underlying mechanisms.Methods Human OSCC cell lines HN6 and Cal27 were treated with 20,40,60,80,100,200 and 400 μM RES,and the effects of RES treatment on the proliferation and migration of HN6 and Cal27 cells were evaluated by CCK-8 and cell scratch assay,respectively;cell cycle was detected by flow cytometry;the concentrations of glucose,pyruvic acid and lactic acid in the supernatant of the cell culture medium were detected by the glycolysis kit;the levels of mRNA expression associated with proliferation,migration and cell cycle were detected by RT-qPCR.Results Compared with the control group,the survival and migration rates of HN6 and Cal27 cells in the RES-treated group were significantly decreased(P＜0.05 or P＜0.01);the RES-treated group induced cell cycle arrest in S phase;in the RES-treated group,the mRNA expression levels of proliferation-related genes,MKi67 and PCNA,S-phase cell cycle-related genes,CyclinA2 and CDK2,and migration-related genes,MMP2,Twist and Snail2 were significantly decreased(P＜0.01);the concentration of glucose in the supernatant of the culture medium of the RES-treated group was significantly higher than that of the control group(P＜0.01),whereas the concentrations of pyruvate and lactate were lower than that of the control group(P＜0.01).Conclusions This study suggests that in vitro treatment of resveratrol can significantly inhibit the proliferation,migration and glycolytic metabolism of OSCC cells,and induce cell cycle arrest in S phase and the down-regulation of proliferation,migration and cycle-related gene expression.
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