Paclitaxel Combined with Anhydrous Ethanol Injection in the Treatment of VX2 Liver Tumor in Rabbits
Objective To investigate the success rate of ultrasound-guided modeling of VX2 liver tumors in rabbits and to evaluate the efficacy of paclitaxel(PTX)combined with anhydrous ethanol injection(AEI)in the treatment of rabbit VX2 liver tumors.Methods VX2 tumor cells were injected into the rabbit livers under ultrasound guidance,and tumor growth was observed using contrast-enhanced ultrasound after 2 weeks.The successfully modeled rabbits were divided into 6 groups:the sham surgery group(injected with 2 mL saline),the clinical AEI-only group(AE dosage was determined according to the volume of the tumor),and 4 groups receiving 2 mLAE combined with different concentrations of PTX[PTX in the order of 0 mg(AEI group),12.5 mg(low-dose PTX group),25.0 mg(medium-dose PTX group),and 37.5 mg(high-dose PTX group)].1 week later,contrast-enhanced ultrasound was performed to evaluate tumor necrosis rate(RNR).After euthanizing the animals,tumor tissue specimens were obtained for HE staining to compare tissue necrosis rate(HNR).Results Ultrasound-guided VX2 rabbit liver tumors were successfully modeled,with in situ tumor formation in the liver(success rate 100%,36/36).PTX dosage was positively correlated with the rate of necrosis(R2=0.946,P＜0.001),and a similar trend was observed for the high necrosis rate(HNR)(R2=0.843,P＜0.001).Correlation analysis showed a significant correlation between PTX dose and tumor necrosis,i.e.,as the PTX concentration increased,the necrosis rate of the tumor also increased(P＜0.001).Conclusion PTX combined with AEI can help to improve the efficacy of treating VX2 liver tumors in rabbits.
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