The Regulatory Effect of miR-296-5p on FGF23 During Neovascularization
Objective To investigate the regulatory role of miR-296-5p during neovascularization by modulating fibroblast growth factor 23(FGF23).Methods Human umbilical vein endothelial cells(HUVECs)were induced with vascular endothelial growth factor(VEGF)to construct an in vitro model simulating neovascularization.The expression levels of miR-296-5p in the control group and the neovascularization group were compared using RT-qPCR.3 groups of cells,namely the miR-296-5p overexpressed group,the miR-296-5p underexpressed group,and the control group,were constructed through cell transfection.The migration and vascularization of these 3 groups were compared,and the expression levels of FGF23 in the 3 groups were compared using Western blot.Results Compared with the untreated control group,the expression level of miR-296-5p was significantly increased in the VEGF-induced neovascularization group(P＜0.001).Compared with the control group,the migration distance and the number of tubes formed were significantly reduced in the miR-296-5p underexpressed group(P＜0.001 and P＜0.05),while they were significantly increased in the miR-296-5p overexpressed group(P＜0.001 and P＜0.01).Compared with the control group,the expression level of FGF23 was significantly decreased in the miR-296-5p underexpressed group(P＜0.01),while it was significantly increased in the miR-296-5p overexpressed group(P＜0.01).Conclusion Our findings suggest that miR-296-5p can promote neovascularization by upregulating the expression of FGF23.
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