The Effects of Down-regulating RAD51C Expression on Tumorigenesis and VEGF and NRP-2 Expression in Mouse Ovarian Cancer in Vivo
Objective To investigate the effects of down-regulating RAD51 paralogous gene C(RAD51C)expression on tumorigenesis and the expression of vascular endothelial growth factor(VEGF)and neuropilin-2(NRP-2)in mouse ovarian cancer in vivo.Methods 3 RAD51C interference vectors(SiRNA-47,SiRNA-183 and SiRNA-285)were constructed in A2780 ovarian cancer cells through culture to detect the expression of RAD51C in the cells,and were packaged as lentiviruses to transfect the cells.qPCR was used to verify the transfection effect,and the stable cell line was constructed.A CDX model was established using Balb/c female nude mice,and 18 nude mice were divided into 3 groups:A2780 cell group(Control group),A2780 cell+empty carrier control group(NC group),A2780 cell+RAD51C interference group(Si-RAD51C group),with 6 mice in each group.The Control group was injected with normal saline,the NC group was injected with empty lentivirus 50 μL,and the Si-RAD51C group was injected with RAD51C interfering lentivirus 50 μL.The tumor composition was observed,and the tumor tissues were taken,and the protein expressions of RAD51C,VEGF and NRP-2 were detected by Western blot(WB)and immunohistochemistry.Results RT-qPCR suggested that the transfection effect of RAD51C interfered with lentivirus was most pronounced with SiRAN-285(P＜0.05).Compared with Control group and NC group,the tumor volume and weight of Si-RAD51C group were the smallest,and the protein expressions of RAD51C,NRP-2 and VEGF were significantly decreased(P＜0.05).Conclusion RAD51C interfered with lentivirus inhibited tumorigenesis in mouse A2780 ovarian cancer cells,and inhibited the expression of RAD51C,NRP-2 and VEGF.
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