In Vitro Drug Release and Mechanical Testing of Imipenem Cilastatin Antibiotic-loaded Bone Cement
Objective To explore the in vitro release rate and antibacterial effect of imipenem cilastatin antibiotic-loaded bone cement sustained-release carriers with different concentrations,as well as their impact on the mechanical properties of bone cement,in order to provide experimental evidence for the clinical treatment of chronic osteomyelitis.Method Different concentrations of antibio:tic gradient solutions were prepared for Liquid Chromatograph Mass Spectrometer(LC-MS)detection,and two standard curve equations were obtained for imipenem and cilastatin respectively.Sustained-release carriers for low,medium,and high concentration groups were prepared by using imipenem cilastatin and bone cement at a mass ratio of 1∶40,4∶40,and 8∶40(g∶g),and in vitro release tests to detect the release rates were conducted at 1,3,7,14,21,28,and 35 days.Antibiotic bone cement with low,medium,and high concentration groups was used to conduct antibacterial tests on Staphylococcus aureus,Escherichia coli,and Pseudomonas aeruginosa,and the diameter of the antibacterial rings were recorded at 1,2,3,4,5,and 6 days.In addition,the compressive strength of bone cement with different concentrations of antibiotics was tested.Result The standard curve equations for imipenem and cilastatin obtained in this experiment were Y=0.001X-0.008 8(R2=0.998 9)and Y=0.002 6X+0.028 1(R2=0.999 9),respectively.At different time points,the release rates of imipenem cisplatin bone cement were smaller in the low concentration group than in the medium concentration group,and smaller in the medium concentration group than in the high concentration group,with statistically significant differences(P＜0.001).The diameters of the antibacterial ring against Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa were shorter in the low concentration group than in the medium concentration group,and shorter in the medium concentration group than in the high concentration group,and the difference was statistically significant(P＜0.05).As the amount of imipenem cilastatin added increased,the compressive strength of bone cement gradually decreased(P＜0.05).Conclusion The prepared imipenem-cilastatin bone cement was observed to exhibit a faster release rate and a more enhanced antibacterial effect with the increase of imipenem-cilastatin concentration,but its compressive strength decreased.
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