Study on Quality Control Methods of Two Different Herba Pteridis Multifidae Based on the Same Efficacy
Objective:To prove the efficacy of two different Herba Pteridis Multifidae through efficacy experiments,and the quality control.Methods:The in vitro cellular inflammation model was established by stimulating RAW 264.7 with LPS and incubated with different concentrations of Pteris cretica L.var.nervosa and Pteris multifida Poir extract.Acute liver injury in ICR mice was induced by intraperitoneal injection of LPS(50 μg/kg)and D-gal(400 mg/kg),and was intervened by intragastric administration of the total extracts of the two medicinal materials.The production of NO in cells,the levels of liver enzymes ALT and AST in serum,the levels of inflammatory factors IL-6 and TNF-α and the activities of oxidative stress factors MDA and SOD in liver homogenate were measured,and the histopathological changes of liver were observed to evaluate its protective effect on acute liver injury induced by LPS/D-gal in mice.The character,microscope and TLC were identified,and the moisture,total ash,acid-insoluble ash and alcohol-soluble extract were determined according to the detection method of Chinese Pharmacopoeia(2020 edition).Luteolin-7-O-rutinoside and luteolin-7-O-glucoside were determined by high performance liquid chromatogrhy(HPLC).Results:The characteristics of Pteris cretica L.var.nervosa and Pteris multifida Poir,the cross section of rhizome and leaf and the microscopic characteristics of powder,and the characteristics of thin layer chromatography pterosin C were determined,and it was stipulated that the moisture content of Herba Pteridis Multifidae should not exceed 10.9%,and the total ash content should not exceed 15.0%.The acid-insoluble ash content should not exceed 10.0%,and the alcohol-soluble extract should not be less than 16.5%.The content limit of quality markers of Herba Pteridis Multifidae was determined,and the determination methods of luteolin-7-O-rutinoside and luteolin-7-O-glucoside were established.The method is well separated and has a good linear relationship in the range of 0.078 2-8.867 8 mg/g and 0.112 2-2.997 1 mg/g,and the average recovery is 104.02%and 92.49%,respectively.Conclusion:The method has strong specificity and good repeatability,and can be used for the quality control of Herba Pteridis Multifidae.
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