Mechanism of Calycosin in the Treatment of Spinal Cord Injury in Rats
Objective:To explore the potential mechanism of Calycosin in the treatment of Spinal Cord Injury.Methods:Thirty rats were randomly divided into Sham group,model group and Calycosin group,ten rats in each group.After spinal cord injury model was established by modified Allen heavy hit method in model group and Calycosin group,rats in the Calycosin group were given 20 mg/kg calycosin by gavage.After the 5 weeks,the modified Tarlov score,cortical somatosensory evoked potential latency time and inclined plate test were measured to determine the spinal cord neurological function of SCI rats.The 5 weeks after modeling,the injury of spinal cord tissues were observed by HE staining.The protein expressions of p-Akt,gp130 and IL-6 in spinal cord were detected by Western blotting.Results:Compared with the Sham group,the modified Tarlov score and the critical angle of tilt plane in the model group were significantly decreased(P<0.05),and the latency time of cortical somatosensory evoked potential wave and the expression of p-Akt,gp130 and IL-6 proteins were significantly increased(P<0.05).Compared with the model group,after Calycosin intervention,the modified Tarlov score and the critical angle of inclined plane were significantly increased(P<0.05),and the latency time of cortical somatosensory evoked potential wave and the expression of p-Akt,gp130 and IL-6 proteins were significantly decreased(P<0.05).Conclusion:Calycosin promoted the recovery of neurological function after SCI in rats by inhibiting the protein expression of gp130 and IL-6 and phosphorylation of PI3K/Akt signaling pathway.