Investigation on the Prescription and Preparation Technology of Puerarin Nanoparticles
Objective:To investigate the formulation and preparation process of phenyl borated chitosan-loaded Puerarin nanoparticles(PBACS-TPP-Pur-NPs).Methods:PBACS-TPP-Pur-NPs were prepared by ion gelation method.With the particle size,PDI,potential,encapsulation efficiency and drug loading rate as evaluation indicators.The factors investigated mainly included:degree of substitution of 3-carboxyphenyl borated chitosan(PBACS),concentration of PBACS,concentration of sodium tripolyphosphate(TPP),pH of PBACS,stirring speed and stirring time.The optimal formulation process was screened out,and the basic properties and in vitro drug release performance of the optimized nanoparticles were characterized and evaluated.Results:The optimized PBACS-TPP-Pur-NPs had moderate and uniform formulation process for preparing PBACS-TPP-Pur-NPs was as follows:the concentration of PBACS was 1.5 mg/mL,the concentration of TPP was 1.4 mg/mL,the pH of modified chitosan was 5.5,the stirring speed was 390.0 r/min,and the stirring time was 30 min.The prepared PBACS-TPP-Pur-NPs have moderate and uniform particle size,strong drug loading capacity,good stability,and obvious slow-release characteristics.Conclusion:The method is effective and feasible,which is suitable for optimizing the formulation and preparation process of Puerarin loaded nanoparticles,and provides a stable basis for the development of Puerarin nanoparticles.
Chitosan3-carboxyl Phenylboronic AcidPuerarinNanoparticlesPreparation Process
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