柏益前列康对非细菌性前列腺炎大鼠NF-кB信号通路的影响
Effect of Baiyi Qianliekang Granules on NF-кB Signaling Pathway in Nonbacterial Prostatitis Rats
刘小军 1王珑 1赵静 1赵辉平 1王俊琴 1李雨容1
作者信息
- 1. 西安新润药业有限公司药物研发中心 西安 710003
- 折叠
摘要
目的:探讨柏益前列康颗粒(BQG)对角叉菜胶所致慢性非细菌性前列腺炎大鼠NF-κB信号通路的影响.方法:1%角叉菜胶复制慢性非细菌性前列腺炎大鼠模型,评估BQG对大鼠前列腺器官指数、组织病理形态以及大鼠前列腺组织中炎症因子IL-8、TNF-α及VEGF表达水平的影响,Western blot检测前列腺组织中NF-κB通路相关分子蛋白表达水平.结果:BQG可以降低前列腺器官指数,减轻前列腺组织内炎症反应.与模型组相比,BQG显著降低前列腺组织中炎症因子IL-8、TNF-α、VEGF表达水平及转录因子p-IκKα、p-IκBα、NF-κBP65、p-NF-κBP65 蛋白表达水平(P<0.05).结论:BQG可抑制角叉菜胶所致大鼠慢性非细菌性前列腺炎,其作用机制可能与抑制NF-кB信号通路有关.
Abstract
Objective:To investigate the NF-кB signaling pathway in Baiyi Qianliekang granule(BQG)in nonbacterial prostatitis rats induced by carrageenan.Methods:Chronic nonbacterial prostatitis rat model was induced by 1%carrageenan.The effects of BQG on rat prostate organ index,histopathological morphology,as well as expression levels of inflammatory factors IL-8,TNF-α,VEGF in rat prostate tissue were evaluated.Western blot was used to detect the expression of NF-κB pathway-related molecular proteins in rat prostate tissues.Results:BQG could reduce prostate organ index and reduces inflammation in prostate tissue.Compared with the model group,the expression levels of inflammatory factors IL-8,TNF-α,VEGF and the expression levels of transcription factors p-IκBα,p-IκKα,NF-κBP65,p-NF-κBP65 in prostate tissue were significantly decreased(P＜0.05).Conclusion:BQG can inhibit chronic nonbacterial prostatitis rats induced by carrageenan,and this effect may be related to inhibiting the activation of NF-кB signaling pathway.
关键词
柏益前列康颗粒/慢性非细菌性前列腺炎/炎症因子/NF-κB信号通路Key words
Baiyi Qianliekang Granule/Chronic Nonbacterial Prostatitis/Inflammatory Factors/NF-кB Signaling Pathway引用本文复制引用
基金项目
陕西省重点研发计划(2022YWZX-03)
出版年
2024
国家科技期刊平台
NSTL
万方数据