摘要
目的:探讨复方丹参滴丸对心血瘀阻型急性心肌梗死(AMI)患者外周血单核细胞自噬因子的干预及对血管内皮功能的保护作用.方法:纳入 2021 年 1 月 1 日—10 月 30 日在江门市五邑中医院心病科诊断为心血瘀阻型AMI患者 60 例,采用前瞻性随机区组法将其分为观察组、对照组及基础组,各 20 例.基础组予西医常规治疗(包括经皮冠状动脉介入术),观察组在西医常规治疗基础上予口服复方丹参滴丸,对照组在西医常规治疗基础上给予丹参酮ⅡA磺酸钠,3 组均治疗 1 周.留取治疗前后外周血标本,通过荧光实时定量PCR法检测p62、Beclin-1、ET-1 的表达,采用酶联免疫吸附法检测vWF、VEGF、LAMP-2 表达水平.结果:治疗前 3 组患者vWF、VEGF、ET-1、LAMP-2、p62、Beclin-1 水平均明显升高,治疗后3 组患者上述指标均显著下降(P<0.01);与基础组相比,观察组及对照组vWF、VEGF、LAMP-2、p62、Beclin-1、ET-1水平更低(P<0.01);与对照组相比,观察组vWF表达水平更低(P<0.01).结论:复方丹参滴丸能抑制心血瘀阻型AMI患者外周血单核细胞自噬反应,并能保护血管内皮功能.
Abstract
Objective:To explore the intervention of compound Danshen dropping pills on autophagy of peripheral blood mononuclear cells in patients with acute myocardial infarction of heart blood stasis type and its protective effect on vascular endothelial function.Methods:From January 1,2021,to October 30,2021,60 patients with acute myocardial infarction of heart blood stasis type diagnosed in the cardiology department of Wuyi traditional chinese medicine hospital in Jiangmen city were included.They were divided into treatment group,control group,and base group by prospective randomized block method,with 20 patients in each group.The primary group was given routine western medicine treatment(including percutaneous coronary intervention),the treatment group was given compound Danshen dropping pills based on conventional western medicine treatment,and the control group was assigned sodium tanshinone IIA sulfonate based on traditional western medicine treatment for one week.Peripheral blood samples were collected before and after treatment,and the expressions of p62,Beclin-1 and ET-1 were detected by fluorescent real-time quantitative PCR,and the expression levels of vWF,VEGF,and LAMP-2 were detected by enzyme-linked immunosorbent assay.Results:The levels of vWF,VEGF,ET-1,LAMP-2,p62,and Beclin-1 in patients in the three groups were significantly increased before treatment,and after treatment,the indexes of the patients in the three groups were significantly decreased(P<0.01);Compared with the basic group,the levels of vWF,VEGF,LAMP-2,p62,Beclin-1 and ET-1 in the treatment group and the control group decreased significantly(P<0.01);Compared with the control group,the expression level of vWF in the treatment group decreased significantly(P<0.01).Conclusion:Compound Danshen dropping pills can inhibit the autophagy of peripheral blood mononuclear cells in patients with acute myocardial infarction of heart blood stasis type and protect vascular endothelium function.
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