Metabolism Characteristics Research of Traditional Chinese Medicine Ingredients Palmatine in Five Species Liver Microsomes
Objective:To study in vitro metabolites of Palmatine in liver microsomes are different among of five species.Methods:Using liver microsome incubation method in vitro,UPLC-Q-TOF-MS with positive ion mode was established and applied to identify the metabolites of Palmatine in liver microsomes of dog(DLM),human(HLM),mice(MLM),monkey(MKLM)and rat(RLM),mobile phase was 0.1％ammonium formate and acetonitrile for gradient elution.Results:A total of 11 metabolites,including 3 metabolites in DLM,8 metabolites in HLM,2 metabolites in MLM,3 metabolites in MKLM and 4 metabolites in RLM,were proposed and identified in liver microsomes of five species in vitro respectively,the metabolic pathway of Palmatine in liver microsomes were demonstrated as hydroxylation,dehydrogenation and hydroxylation,dehydrogenation and demethylation,demethylation and hydroxylation.,Conclusion:There are some differences in liver microsomes metabolism between species.Furthermore,the present study provided vital data for further pharmacokinetic and in vivo metabolism study of palmatine.
万方数据
维普
