Dihydroflavone Sikokianin E Increases PPAR y Regulating the PI3K/Akt/GLUT4 Pathway for Hypoglycemic and Weight Loss Effects in db/db Mice
Objective:To explore the hypoglycemic and weight-reducing effects of Sikokianin E in type 2 diabetes model db/db miceo Methods:The glucose tolerance and insulin sensitivity of Sikokianin E in db/db mice were evaluated by oral glucose tolerance test(OGTT),intraperitoneal insulin tolerance test(IPGTT)and calculation of the area under the curve(AUC),by Elisa method Serum insulin levels were measured to evaluate the insulin resistance level of mice,glycated hemoglobin(HbA1c)levels were detected by immunoturbidimetry,and four serum lipids(TC,TG,LDL-c and HDL-c)were measured by chemiluminescence kit.and liver function levels(ALT and AST).HE staining was used to observe the liver morphology of mice.Western blot was used to examine the relative protein expressions of PPAR γ,PI3K,p-PI3K,Akt,p-Akt and GLUT4.quantity.Results:Compared with the model group,the 28 day body weight,average food intake and fasting blood glucose of the Sikokianin E group were significantly reduced(P<0.05);compared with this group before intervention,the AUC of OGTT and IPGTT in the Sikokianin E group were significantly reduced after intervention(P<0.05);it can also significantly reduce serum TC,TG,ALT and AST levels(P<0.05);Sikokianin E group can significantly increase the relative protein expression of liver PPAR γand GLUT4,significantly increase p-PI3K/PI3K and p-Akt/Akt ratio(phosphorylation degree)(P<0.05,P<0.01);liver HE staining results showed that Sikokianin E improved hepatic steatosis in db/db mice.Conclusion:The hypoglycemic and weight-loss effects of Sikokianin E in db/db mice are related to improving blood glucose homeostasis,increasing insulin resistance and regulating blood lipids.Its mechanism of action may through increasing PPAR γ and activating the PI3K/Akt/GLUT4 signaling pathway.
Sikokianin EReduce Blood Sugar and Weight LossPharmacological Mechanism