Study on the Substitution of Resina Draconis for Draconis Sanguis in Shexiang Jiegu Capsules
Objective:Take loureirin A and loureirin B as specific targets,to establish a detection method to identify the substitution of resina draconis for draconis sanguis in Shexiang Jiegu Capsules.Methods:Qualitative analysis of the 3%phosphoric acid methanol solution extract of the prescription formulation was performed using HPLC-UV method,and then qualitative validation was performed using UPLC-MS/MS method.Results:The established HPLC-UV method for the determination of loureirin A and loureirin B showed good linearity,with recoveries of 98.23%and 98.81%,and RSDs of 1.41%and 0.89%,respectively.The UPLC-MS/MS method has good specificity,high sensitivity,and can accurately verify detection.In addition,after analysis and determination,6 out of 26 batches of samples were tested for loureirin A and loureirin B components,and the unqualified rate was 23%,indicating that the raw material of draconis sanguis was mixed with fake resina draconis.Conclusion:This method is simple,fast,and accurate,and can be used for the inspection and analysis of draconis sanguis as a substitute for resina draconis in Shexiang Jiegu Capsules.
Shexiang Jiegu CapsuleResina DraconisDraconis SanguisLoureirin ALoureirin B
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