HPLC法同时测定不同地区大麻籽油中大麻二酚与Δ9-四氢大麻酚的含量
Simultaneous Determination of Cannabidiol and Δ9-Tetrahydrocannabinol in Hemp Seed Oils from Different Regions by HPLC Method
杨欣 1易丽娟 1戴娟 1金晓飞2
作者信息
- 1. 湖南中医药高等专科学校 湖南 株洲 412012
- 2. 湖南麓山生物研究院有限责任公司 长沙 410208
- 折叠
摘要
目的:研究建立一套高效液相色谱法,测定大麻籽油中大麻二酚(CBD)与四氢大麻酚(Δ9-THC)的含量,并以此作为考察依据分析不同产地大麻籽成分情况.方法:以TOSOH Amide-80(4.6 mm×250 mm,5 μm)为色谱柱,蒸发光散射检测器雾化管温度为 80℃;漂移管温度为 100℃,气体流速 1.6 L/min,以乙腈-水(80∶20)为流动相,流速为1.0 mL/min,建立HPLC测定CBD和Δ9-THC含量的方法.结果:该方法的LOQ、线性范围和精密度均能满足对油脂类样品中CBD和Δ9-THC检测要求,所检测的 9 个地区的工业大麻籽油中CBD含量为 0.014%～0.117%,Δ9-THC含量为0.375%～0.022%.结论:该方法简便准确,可用于不同地区大麻子油的质量标准研究提供参考.
Abstract
Objective:To establish a high-performance liquid chromatography method for determining the content of cannabidiol(CBD)and Δ9-tetrahydrocannabinol(Δ9-THC)in hemp seed oil,and to use it as a basis for analyzing the composition of hemp seeds from different regions.Methods:TOSOH Amide-80(4.6 mm×250 mm,5 μm)was used as the chromatographic column,and the atomization tube temperature of the evaporative light scattering detector was 80℃;The drift tube temperature is 100℃,the gas flow rate is 1.6 L/min,acetonitrile water(80∶20)is used as the mobile phase,and the flow rate is 1.0 mL/min.An HPLC method is established to determine the content of CBD and Δ9-THC.Results:The LOQ,linear range,and precision of this method can meet the requirements for detecting CBD and Δ9-THC in oil samples.The CBD content in industrial hemp seed oil from the 9 regions tested ranges from 0.014%to 0.117%,and the Δ9-THC content ranges from 0.375%to 0.022%.Conclusion:This method is simple and accurate,and can provide reference for the quality standard research of hemp seed oil in different regions.
关键词
工业大麻/大麻二酚/四氢大麻酚/CBD/Δ9-THC/HPLCKey words
Industrial Hemp/Cannabidiol/Tetrahydrocannabinol/CBD/Δ9-THC/HPLC引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据