Regulation of Electrothermal Acupuncture at Sanyinjiao on Ovarian Reserve Function in Rats with Premature Ovarian Insufficiency
Objective:To observer the regulation of electrothermal acupuncture therapy at Sanyinjiao on ovarian reserve function in rats with premature ovarian insufficiency(POI),provide scientific basis for the clinical application of Sanyinjiao.Methods:Thirty-two SPF female SD rats with regular estrous cycle were selected and randomly divided into blank group,model group,Sanyinjiao electrothermal acupuncture group and non-acupoint electrothermal acupuncture group,with 8 rats in each group(n=8).The blank group was given 0.9%physiological saline by gastric irrigation,and the model group was given Tripterygium glycosides tablet suspension by gastric irrigation.The next day after the end of the model,electrothermal acupuncture intervention was started,Sanyinjiao group took Sanyinjiao points,the non-meridian and non-acupoints were selected in the non-acupoint group.Both groups are connected with the electrothermal acupuncture instrument,continuous wave,frequency was 2 Hz,20 minutes each time,once a day,continuous intervention 14 days.The blank group and the model group were given the same time of capture and fixation as the electric temperature needle group at the same time,without any treatment.After the intervention,samples were collected from each group of rats during the diestrus period,the ELISA method was applied to test the level of follicle-stimulating hormone(FSH),estradiol(E2),anti-mllerian hormone(AMH)and inhibin B(INHB);The morphological changes of ovarian in each group tissue were observed by HE staining.Results:Compared with the blank group,the ovary index,serum E2,AMH,INHB reduced,while serum FSH increased in the model group(P<0.01).Compared with the model group,the ovary index,serum AMH,E2,INHB significantly increased,while serum FSH significantly reduced in the Sanyinjiao group,the differences were statistically significant(P<0.05,P<0.01);serum AMH increased in the non-acupoint group(P<0.01).Compared with the non-acupoint group,the ovary index,serum AMH,E2,INHB,FSH significantly changed in the Sanyinjiao group,the differences were statistically significant(P<0.05,P<0.01).Conclusion:(1)Sanyinjiao electrothermal acupuncture can regulate the levels of sex hormones in POI model rats and improve the reproductive endocrine environment;(2)Sanyinjiao electrothermal acupuncture can improve ovarian reserve function in POI model rats,which is likely to be related with regulating serum hormone and AMH levels.
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