摘要
目的:建立超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定沙苑子中11种黄酮类成分(杨梅素-3-O-β-D-葡萄糖苷、沙苑子苷A、沙苑子苷B、紫云英苷、毛蕊异黄酮葡萄糖苷、鼠李柠檬素-3-O-β-D-葡萄糖苷、毛蕊异黄酮、异槲皮苷、番石榴苷、芒柄花素、鼠李柠檬素)的含量.方法:采用Waters ACQUITY UPLC BEH C18 柱(100 mm×2.1 mm,1.7 μm);以甲醇和0.1%甲酸水溶液为流动相,梯度洗脱,流速0.2 mL/min;柱温40℃;进样量为1 μL;电喷雾离子化正、负离子模式,多反应监测模式.结果:在考察的浓度范围内,11种黄酮类成分线性关系良好(r≥0.999 55);平均加样回收率和RSD分别为 97.63%～102.87%和 0.96%～2.73%.结论:该方法准确度、精密度、稳定性、线性关系良好,可用于沙苑子中 11 种黄酮类成分的含量测定.
Abstract
Objective:To establish a method for simultaneous determination of 11 flavonoid components(myricetin-3-O-β-D-glucopyranoside,complanatoside A,complanatoside B,astragalin,calycosin-7-O-β-D-glucoside,rhamnocitrin 3-glucoside,calycosin,isoquercitrin,guaijaverin,formononetin,rhamnocitrin)in Astragali Complanati Semen by ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Methods:Using Waters ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm),methanol and 0.1%formic acid aqueous solution as mobile phases,gradient elution was performed at a flow rate of 0.2 mL/min.Column temperature was 40℃and injection volume was 1 μL.The positive and negative ion modes and the multi reaction monitoring mode were adopted for the electro spray ionization.Results:Within the concentration range under investigation,there was a good linear relationship(r≥0.999 55)among the 11 flavonoid components;The average sample recovery rate and RSD were ranged from 97.63%-102.87%and 0.96%-2.73%.Conclusion:This method has good accuracy,precision,stability,and linear relationship,and can be used for the determination of eleven flavonoid components in Astragali Complanati Semen.
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