Effect of Trans-3,4,5-trihydroxystilbene on Protection Research in AGEs-induced H9C2 Myocardial Cell Apoptosis
Objective:To investigate the effect of trans-3,4,5-trihydroxystilbene on H9C2 cardiomyocytes against AGEs-induced apoptosis and potential molecular mechanisms.Methods:H9C2 cardiomyocytes cells cultured in vitro were divided into four groups:normal contol group,AGEs group,AGEs and trans-3,4,5-trihydroxystilbene(RESV)group,AGEs and NAC group.Cell viabillity rate was measured by CCK-8 assay,ROS production was measured by DCFH-DA fluorescent probe.Nucleus morphology was observed under fluorescence microscope after being incubatedwith Honchest 33258;western blotting was applied to assess the apoptotic components expression including Bax and Bcl-2.Results:Compared with control group,cell viability rate in AGEs group were decreased,repectively,in a dose-dependent manner;cell apoptosis and ROS production in H9C2 cells were remarkably increased in AGEs group(P<0.05).However,compared with AGEs group,trans-3,4,5-trihydroxystilbene group reduced ROS productionand ameliorated cell apoptosiscaused by AGEs(P<0.05);the expression of proapotosis proteins Bax was decreased,the expression of anti-apoptosis proteins like Bcl-2 was increased(P<0.05).Conclusion:Trans-3,4,5-trihydroxys tilbenne protect H9C2 cardiomyocytes against AGEs-induced apoptosis,which may be related to the reduction of ROS.
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