Effect of Moxibustion at Guanyuan Acupoint on Kidney Energy Metabolism in Young Rats Based on Metabolomics
Objective:To explore the effect and mechanism of long-term moxibustion at Guanyuan acupoint on kidney energy metabolism of young rats,so as to provide theoretical basis for the rational application of moxibustion in children.Methods:Three-week-old male SD rats were divided into blank control group,Jinguishenqi Pill group and moxibustion group,with 8 rats in each group.The Jinguishenqi Pill group was given daily according to body weight,while the moxibustion group was given moxibustion at Guanyuan acupoint for 15 min daily.After the experiment lasted for 13 weeks,the contents of serum adrenocorticotropin(ACTH),adrenocorticotropin releasing hormone(CRH),corticosterone(CORT),triiodothyronine(T3),thyroxine(T4),testosterone(T)and the activities of succinate dehydrogenase(SDH),malate dehydrogenase(MDH),NADH oxidase(NOX)and adenosine triphosphate(ATP)enzymes in renal tissue were observed,and using metabolomics technology to explore the mechanism of moxibustion.Results:Compared with the blank control group,after moxibustion at Guanyuan acupoint in young rats,the content of ACTH in serum increased(P<0.05),the content of CRH decreased(P<0.01),but the content of CORT,T3,T4 and T did not change significantly(P>0.05).The activity of NOX in kidney decreased significantly(P<0.01),the activity of Na+-K+-ATPase and Ca2+-Mg2+-ATPase increased significantly(P<0.05),but the changes of SDH and MDH were not significant(P>0.05).Metabonomics results showed that two common difference compounds were isolated from kidney tissue and nine common difference compounds were isolated from feces.Conclusion:Moxibustion at Guanyuan acupoint can affect the function of kidney Yang by regulating the activity of mitochondrial NOX and ATPase in kidney tissue of young rats,and the secretion of ACTH and CRH in serum,which may be related to the regulation of vitamin B6 metabolism,α-linolenic acid metabolism,nicotinate and nicotinamide metabolism,porphyrin and chlorophyll metabolism,unsaturated fatty acid biosynthesis and tryptophan metabolism.
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