首页|Coupled single-cell and bulk RNA-seq analysis reveals the engulfment role of endothelial cells in atherosclerosis
Coupled single-cell and bulk RNA-seq analysis reveals the engulfment role of endothelial cells in atherosclerosis
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
维普
万方数据
The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic plaque.Single-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopu-lation in atherosclerosis,which was strongly associated with vascular injury-related pathways.Moreover,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury circumstances.Single-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpop-ulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for phagocytosis.Furthermore,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debhs in atherosclerotic plaque of both mouse and human aorta.Overall,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.
Apoptotic cell debrisAtherosclerosisEndothelial engulfmentSingle-cell RNA sequencingScavenger receptor class B type 1
Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering Modern Life Science Experiment Teaching Center of Bioengineering College,Chongqing University,Chongqing 400030,China
School of Basic Medical Sciences,Chengdu Medical College,Chengdu,Sichuan 610500,China
Chongqing Emergency Medical Center(Chongqing University Central Hospital),Chongqing 400014,China
Jinfeng Laboratory,Chongqing 401329,China
展开 >
National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaScience and Technology Innovation Project of Jinfeng Laboratory,Chongqing,China
维普
万方数据
