首页|m6A Profile Dynamics Indicates Regulation of Oyster Development by m6A-RNA Epitranscriptomes
m6A Profile Dynamics Indicates Regulation of Oyster Development by m6A-RNA Epitranscriptomes
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The N6-methylation of RNA adenosines(N6-methyladenosine,m6A)is an important reg-ulator of gene expression with critical implications in vertebrate and insect development.However,the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown.Using methylated RNA immunoprecipitation sequencing(MeRIP-seq),we generated transcriptome-wide m6A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles.Oyster RNA classes display specific m6A signatures,with messenger RNAs(mRNAs)and long non-coding RNAs(lncRNAs)exhibiting distinct profiles and being highly methy-lated compared to transposable element(TE)transcripts.Epitranscriptomes are dynamic and corre-spond to the chronological steps of development(cleavage,gastrulation,organogenesis,and metamorphosis),with minimal mRNA and IncRNA methylation at the morula stage followed by a global increase.mRNA m6A levels are correlated with transcript levels,and shifts in methylation pro-files correspond to expression kinetics.Differentially methylated transcripts cluster according to em-bryo-larval stages and bear the corresponding developmental functions(cell division,signal transduction,morphogenesis,and cell differentiation).The m6A level of TE transcripts is also regu-lated and peaks during the gastrulation.We demonstrate that m6A-RNA methylomes are dynamic and associated with gene expression regulation during oyster development.The putative epitranscrip-tome implication in the cleavage,maternal-to-zygotic transition,and cell differentiation in a lophotro-chozoan model brings new insights into the control and evolution of developmental processes.
Lorane Le Franc、Bruno Petton、Pascal Favrel、Guillaume Rivière
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Laboratoire de Biologie des Organismes et des Ecosystèmes Aquatiques(BOREA),Muséum d'Histoire Naturelle,Sorbonne Université,Université de Caen Normandie,Université des Antilles,CNRS UMR 8067,IRD,14032 Caen,France
Ifremer,Laboratoire des Sciences de l'Environnement Marin,UMR 6539 CNRS/UBO/IRD/Ifremer,Centre Bretagne,29280 Plouzané,France
French national program CNRS EC2CO ECODYNCouncil of the Normandy Region(RIN ECUME)
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