Characteristics of N6-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii

扫码查看

原文链接

万方数据

外文摘要：The unicellular green alga Chlamydomonas reinhardtii(hereafter Chlamydomonas)pos-sesses both plant and animal attributes,and it is an ideal model organism for studying fundamental processes such as photosynthesis,sexual reproduction,and life cycle.N6-methyladenosine(m6A)is the most prevalent mRNA modification,and it plays important roles during sexual reproduction in ani-mals and plants.However,the pattern and function of m6A modification during the sexual repro-duction of Chlamydomonas remain unknown.Here,we performed transcriptome and methylated RNA immunoprecipitation sequencing(MeRIP-seq)analyses on six samples from different stages during sexual reproduction of the Chlamydomonas life cycle.The results show that m6A modifica-tion frequently occurs at the main motif of DRAC(D=G/A/U,R=A/G)in Chlamydomonas mRNAs.Moreover,m6A peaks in Chlamydomonas mRNAs are mainly enriched in the 3'untrans-lated regions(3'UTRs)and negatively correlated with the abundance of transcripts at each stage.In particular,there is a significant negative correlation between the expression levels and the m6A levels of genes involved in the microtubule-associated pathway,indicating that m6A modification influences the sexual reproduction and the life cycle of Chlamydomonas by regulating microtubule-based movement.In summary,our findings are the first to demonstrate the distribu-tion and the functions of m6A modification in Chlamydomonas mRNAs and provide new evolution-ary insights into m6A modification in the process of sexual reproduction in other plant organisms.

外文关键词：

N6-methyladenosinem6A sequencingSexual reproductionMicrotubule-associated pathwayPhotosynthesisChlamydomonas reinhardtii

作者：

Ying Lv、Fei Han、Mengxia Liu、Ting Zhang、Guanshen Cui、Jiaojiao Wang、Ying Yang、Yun-Gui Yang、Wenqiang Yang

展开 >

作者单位：

Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China

China National Botanical Garden,Beijing 100093,China

College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

CAS Key Laboratory of Genomic and Precision Medicine,Beijing Institute of Genomics,Chinese Academy of Sciences and China National Center for Bioinformation,Beijing 100101,China

Sino-Danish College,University of Chinese Academy of Sciences,Beijing 100049,China

Institute of Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China

Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100093,China

展开 >

基金：

National Key R&D Program of ChinaNational Key R&D Program of ChinaNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of China

项目编号：

2019YFA09046002018YFA08012002021YFA09108003187021791940304

出版年：

2023

DOI：

10.1016/j.gpb.2022.04.004

基因组蛋白质组与生物信息学报(英文版)

中国科学院北京基因组研究所

基因组蛋白质组与生物信息学报(英文版)

CSTPCDCSCD

影响因子：0.495

ISSN：1672-0229

年,卷(期)：2023.21(4)

参考文献量91