首页|RNA Methylome Reveals the m6A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering

RNA Methylome Reveals the m6A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering

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The epitranscriptomic mark N6-methyladenosine(m6A),which is the predominant inter-nal modification in RNA,is important for plant responses to diverse stresses.Multiple environmen-tal stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m6A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m6A-mediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m6A level in tea leaves were mainly controlled by two m6A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solar-withering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m6A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions.

Camellia sinensisRNA methylationEpitranscriptomeSecondary metaboliteWithering

Chen Zhu、Shuting Zhang、Chengzhe Zhou、Caiyun Tian、Biying Shi、Kai Xu、Linjie Huang、Yun Sun、Yuling Lin、Zhongxiong Lai、Yuqiong Guo

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College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Tea Industry Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Earmarked Fund for China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural AffairsScientific Research Foundation of Graduate School of Fujian Agriculture and Forestry UniversityScientific Research Foundation of Horticulture College of Fujian Agriculture and Forestry UniversityRural Revitalization Tea Industry Technical Service Project of Fujian Agriculture and Forestry Universitythe"Double firstclass"scientific and technological innovation capacity and enhancement cultivation plan of Fujian Agriculture an6.18 Tea Industry Technology Branch of Collaborative Innovation InstituteFujian Agriculture and Forestry University Construction Project for Technological Innovation and Service System of Tea IndusConstruction of Plateau Discipline of Fujian ProvinceTea Industry Branch of Collaborative Innovation Institute of Fujian Agriculture and Forestry UniversitySpecial Fund for Science and Technology Innovation of Fujian Zhang Tianfu Tea Development Foundation,China

CARS-19324-1122yb0702019B0111899170145KSYLP004K1520001AK1520005A01102/71201801101K1521015AFJZTF01

2023

10.1016/j.gpb.2023.02.003

基因组蛋白质组与生物信息学报(英文版)
中国科学院北京基因组研究所

基因组蛋白质组与生物信息学报(英文版)

CSTPCDCSCD
影响因子：0.495
ISSN：1672-0229
年,卷(期)：2023.21(4)
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