首页|Dynamic DNA 5-hydroxylmethylcytosine and RNA 5-methycytosine Reprogramming During Early Human Development

Dynamic DNA 5-hydroxylmethylcytosine and RNA 5-methycytosine Reprogramming During Early Human Development

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After implantation,complex and highly specialized molecular events render functionally distinct organ formation,whereas how the epigenome shapes organ-specific development remains to be fully elucidated.Here,nano-hmC-Seal,RNA bisulfite sequencing(RNA-BisSeq),and RNA sequencing(RNA-Seq)were performed,and the first multilayer landscapes of DNA 5-hydroxymethylcytosine(5hmC)and RNA 5-methylcytosine(m5C)epigenomes were obtained in the heart,kidney,liver,and lung of the human foetuses at 13-28 weeks with 123 samples in total.We identified 70,091 and 503 organ-and stage-specific differentially hydroxymethylated regions(DhMRs)and m5C-modified mRNAs,respectively.The key transcription factors(TFs),T-box transcription factor 20(TBX20),paired box 8(PAX8),krueppel-like factor 1(KLF1),transcription factor 21(TCF21),and CCAAT enhancer binding protein beta(CEBPB),specifically contribute to the formation of distinct organs at different stages.Additionally,5hmC-enriched Alu elements may participate in the regulation of expression of TF-targeted genes.Our integrated studies reveal a putative essential link between DNA modification and RNA methylation,and illustrate the epige-netic maps during human foetal organogenesis,which provide a foundation for an in-depth understanding of the epigenetic mechanisms underlying early development and birth defects.

Human foetusFoetal organogenesisDNA 5hmCRNA m5CPost-transcriptional regulation

Xiao Han、Jia Guo、Mengke Wang、Nan Zhang、Jie Ren、Ying Yang、Xu Chi、Yusheng Chen、Huan Yao、Yong-Liang Zhao、Yun-Gui Yang、Yingpu Sun、Jiawei Xu

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Center for Reproductive Medicine,Henan Key Laboratory of Reproduction and Genetics,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

Key Laboratory of Genomic and Precision Medicine,Beijing Institute of Genomics,Chinese Academy of Sciences and China National Center for Bioinformation,Beijing 100101,China

University of Chinese Academy of Sciences,Beijing 100049,China

Sino-Danish College,University of Chinese Academy of Sciences,Beijing 100049,China

Institute of Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China

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National Key R&D Program of ChinaNational Key R&D Program of ChinaNational Key R&D Program of ChinaNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaScientific and Technological Innovation Talent Project of Universities of Henan Province,ChinaShanghai Municipal Science and Technology Major Project,ChinaChina Postdoctoral Science Foundation

2019YFA01109002019YFA08022022019YFA08022002020YFA0803401318708173217081920HASTIT0452017SHZDZX012021M692927

2023

10.1016/j.gpb.2022.05.005

基因组蛋白质组与生物信息学报(英文版)
中国科学院北京基因组研究所

基因组蛋白质组与生物信息学报(英文版)

CSTPCDCSCD
影响因子：0.495
ISSN：1672-0229
年,卷(期)：2023.21(4)
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