首页|NSUN2-mediated mRNA m5C Modification Regulates the Progression of Hepatocellular Carcinoma

NSUN2-mediated mRNA m5C Modification Regulates the Progression of Hepatocellular Carcinoma

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RNA modifications affect many biological processes and physiological diseases.The 5-methylcytosine(m5C)modification regulates the progression of multiple tumors.However,its char-acteristics and functions in hepatocellular carcinoma(HCC)remain largely unknown.Here,we found that HCC tissues had a higher m5C methylation level than the adjacent normal tissues.Transcriptome analysis revealed that the hypermethylated genes mainly participated in the phosphokinase signaling pathways,such as the Ras and PI3K-Akt pathways.The m5C methyltransferase NSUN2 was highly expressed in HCC tissues.Interestingly,the expression of many genes was positively correlated with the expression of NSUN2,including GRB2,RNF115,AATF,ADAM 15,RTN3,and HDGF.Real-time PCR assays further revealed that the expression of the mRNAs of GRB2,RNF115,and AATF decreased significantly with the down-regulation of NSUN2 expression in HCC cells.Furthermore,NSUN2 could regulate the cellular sensitivity of HCC cells to sorafenib via modulating the Ras signaling pathway.Moreover,knocking down NSUN2 caused cell cycle arrest.Taken together,our study demonstrates the vital role of NSUN2 in the progression of HCC.

5-methylcytosineHepatocellular carcinomaNSUN2Ras pathwaySorafenib

Dan Song、Ke An、Wenlong Zhai、Luyao Feng、Yingjie Xu、Ran Sun、Yueqin Wang、Yun-Gui Yang、Quancheng Kan、Xin Tian

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Department of Pharmacy,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

CAS Key Laboratory of Genomic and Precision Medicine,Beijing Institute of Genomics,Chinese Academy of Sciences and China National Center for Bioinformation,Beijing 100101,China

Henan Key Laboratory of Precision Clinical Pharmacy,Zhengzhou University,Zhengzhou 450052,China

Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

Key Laboratory of Digestive Organ Transplantation of Henan Province,Zhengzhou University,Zhengzhou 450052,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaProvince and Ministry Coconstruction Major Program of Medical Science and Technique Foundation of Henan ProvinceSpecial Fund for Young and Middle School Leaders of Henan Health Commission,China

3217059431870809SBGJ202001007HNSWJW-2020017

2023

10.1016/j.gpb.2022.09.007

基因组蛋白质组与生物信息学报(英文版)
中国科学院北京基因组研究所

基因组蛋白质组与生物信息学报(英文版)

CSTPCDCSCD
影响因子：0.495
ISSN：1672-0229
年,卷(期)：2023.21(4)
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