Optimization of Extracting Conditions and the Content Comparative Analysis of Flavonoid of Ardisia corymbifera Mez var. tuberifera C. Chen from Different Areas and Different Sections
In this research, we established a method of quantity determination of total flavonoid in Ardisiacorymbijera Mez var. tuberifera C. Chen and comparative analysed content differences of flavonoid in different sections A . var. tuberifera from different areas by orthogonal experiment and spectrophotometry. The results indicated the optimum extractive technology of determination of total flavonoid in A . var. tuberifera by spectrophotometry was that extraction reagent 60％ alcohol, ratio of liquid/solid as 1:70, extracting power 200 W, extracting temperature 70℃ and extracting time 50 min. On the other hand, the quantitative order of flavonoid of A. var. tuberifera from different areas was that Jingxi＞>Napo＞Debao＞Fusui＞Longzhou＞Wuming as well as the quantitative order of flavonoid in different sections of A. var. tuberifera from the same area was leaf＞root＞stem. The optimum extract condition was detected by the experiment of precision, repeatability, stability and recovery, The results displayed that this method was simple, accurate, repeatable, the RSD was 0.788％, stable and high recovery with the average recovery of 96.20％ , which showed that this method was feasibling for comparative content differences of flavonoid in different sections A . var. tuberifera from different areas and would provide some important direction for its quality analysis.
NETL
NSTL
万方数据
维普
