Comparison on the Dehydration Protection Systems and Signal Transduction Relationship of CaCl2 and ABA Pretreatment on the Phalaenopsis PLB
In this paper we pretreated Phalaenopsis PLB (protocorm-like body) with CaCl2, ABA and both, respectively, and compared survival rate, dehydration protection system before and after desiccation, as well as the signal transduction relationship between Ca2+ and ABA. The results showed survival rates of dehydrated PLB pretreated with CaCl2, ABA and both all increased significantly. CaCl2 and ABA exhibited similar impact tendencies on the indexes of soluble carbohydrate, sucrose, reducing carbohydrate, SOD activity and antioxidative capability, respectively, but with respect to the indexes of soluble protein, heat-stable protein, POD and CAT, their impact tendencies showed deviation. The ability that ABA increased the dehydration tolerance of PLB was weaken by promethazine (calmodulin antagonist) or BAPTA/AM (cytosolic calcium chelator). Ancymidol (inhibitor of ABA synthesis) decreased the effect of ABA on the PLB's dehydration tolerance. Due to the fact that the effects of CaCl2 and ABA had almost the same basic of physiology and biochemistry, and that ABA and Ca2+ related to each other by the signal transduction system during dehydration, it is practical to replace ABA with CaCl2 for pretreatment to induce the dehydration tolerance of Phalaenopsis PLB.
Phalaenopsis PLB, Ca2+, ABA, Pretreatment, Dehydration protection system, Signal transduction
NETL
NSTL
万方数据
维普
