摘要
本文建立高效的亮菌多糖分离纯化工艺，并研究其体内抗肿瘤活性。以市售亮菌粗粉为原料，采用水提醇沉、Sevag法脱蛋白、DEAE纤维素离子交换层析、Sephadex G-100凝胶过滤层析等方法得到亮菌多糖ATPSⅡ和ATPSⅡ-2；建立小鼠S180实体瘤模型，以生理盐水和环磷酰胺为阴性和阳性对照，观察ATPSⅡ和ATPSⅡ-2在不同剂量(10 mg/kg,40 mg/kg,80 mg/kg)给药10 d后，对小鼠肿瘤抑制的作用。结果表明：亮菌粗粉中多糖含量为22.7 g/100 g；紫外光谱分析显示纯化得到的ATPSⅡ纯度接近100%；ATPSⅡ和ATPSⅡ-2对肿瘤的抑瘤率随着多糖浓度的增大而增大，其高剂量组对S180的抑瘤率分别为46.7%和51.7%，与阳性对照组相比，亮菌精制多糖ATPSⅡ和ATPSⅡ-2高剂量组抗S180效果接近于临床化疗药环磷酰胺，具有显著的抗肿瘤活性。
Abstract
Ahigh-efficiencyprocessforisolationandpurificationofpolysaccharidefromArmillariellatabescensas well as the anti-tumor activity in vivo of this polysaccharide were studied in this paper. ATPSII, ATPSII-2, two kinds of A rmillariella tabescens polysaccharide were isolated by ethanol subsiding method, sevag method, DEA E-cellulose ion-exchange chromatograp, and sephdex G-100h gel filtration from commercially available powder of A rmillariella tabescens. A mouse model of S180 solid tumor was established to observe the inhibitory effect on tumor growth in mice. Using the normal saline and the cyclophosphamideas as negative and positive controls, the mice were treated with different dose of Armillariella polysaccharide (10 mg/kg, 40 mg/kg, 80 mg/kg) for 10 days. The results showed that the contents of polysaccharide in crude powder of Armillariella tabescens was 16.7 g/100 g, and the purity of ATPSIIisolated from A rmillariella tabescens in this study was nearly 100%by UV spectraum anaylisis. It was discovered that the tumor inhibitory rate increased with the increases of concentrations of ATPSIIand ATPSII-2, which the tumor inhibitory rate were 46.7%and 51.7%at the highest dose, respectively. The results indicated the highest dose group of the refined ATPSIIand ATPSII-2 showed an obvious effect on inhibiting the growth of mice sarcoma S180, which the anti-tumor activity of them was close to the cyclophosphamideas.
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