Investigation of Morphological Characteristics during Liquid Oscillation Propagation of Dendrobium officinale
D.officinale was widely used as traditional Chinese medicinal materials in China.Because the seeds of D.officinale were very difficult to germinating,tissue culture tum to the main propagations methods.Due to hy-bridization,this method could not keep the characters of the mother plants completely.In this study,we used the protocorm-like bodies (PLBs) induced from in vitro shoot axiliary buds ofD.officinale as explants and established the liquid oscillations rapid propagation system.In this system,the liquid medium was MS+BA 2 mg/L+NAA 0.1 mg/L.The PLBs proliferation rate was 10％～15％ during 2 months and increased to 30％～50％ after 2 months.The PLBs were first oyster white and then turned to reseda and semitransparent.The cluster of PLBs began to germinate after 3～4 months.These PLBs were transplanted to solid medium MS+BA 0.2 mg/L+NAA 0.05 mg/L and the semitransparent PLBs turned to non-transparent green.After the PLBs germinated and developed to shoots,they were transplanted to solid medium MS+NAA 0.1 mg/L+banana 100 g/L to induced to rooting and forming whole seedlings.These results supplied visual reference for rapid propagation of orchids.
D. officinaleProtocorm-like bodiesLiquid oscillation culture
NETL
NSTL
万方数据
维普
