In Silico Cloning and Bioinformatics Analysis of KNOX Gene in Sugarcane (Sckn1)
KNOX plays an important role in the formation and maintenance of plant apical meristem.We chose rice KNOX gene (OSH1,Oskn1) as the reference sequence to clone the KNOX gene that is homologous with OSH1 from sugarcane (named Sckn1) based on in silico cloning approach,then,the structure and function of Sckn1 was analyzed and predicted by bioinformatics.The results showed that Sckn1 contains a 1 071 bp open reading frame encoding 356 amino acids with the molecular weight 39.36 kD and theoretical isoelectric point 6.47.This gene is likely to be located in the nucleus,playing a role of transcriptional regulation.Sequence alignment revealed that ScKN1 and KN1 from sorghum,zea mays,setaria italica should be of great similar.These analyses lay a solid foundation for further research ofSckn1.
NETL
NSTL
万方数据
维普
