Rhizosphere Bacteria Diversity of Morus mongolica Revealed Based on 16S rRNA Sequencing
Plant rhizosphere is rich in variety of bacteria,which have an important influence on the growth and development of plants.In order to better understand the diversity and difference of rhizosphere bacterias between the wild and cultivated Morus mongolica,we extracted the metagenomic DNA and analyzed the rhizosphere microflora of two samples using the Roche 454 sequencing technology.The rhizosphere bacteria were characterized by amplifying the V 1～V3 region of bacterial 16S rRNA gene.The results showed that the dominant taxa in wild Morus mongolica Rhizosphere bacteria are Proteobacteria (31.62％) and Acidobacteria (19.8％),while in the cultivated sample,the dominant taxa were Firmicutes (89.07％).The Shannon index were 5.8 and 1.33 in the wild Morus mongolica rhizosphere bacteria and cultivated one.In cultivated Morus mongolica sample,78.9％ of the total sequence belongs to OTU498,the closest genus of which is Bacillus thuringiensis.The four group of OTU656,OTU556,OTU568 and OTU665 in wild Morus mongolica sample,which accounted for 8.17％ of the total sequence,is similar to the Methylobacterium sp.the rhizosphere bacterias of the two sample belong to the same Phylum,but different genus.The results of this study demonstrated that the diversity of cultivated Morus mongolica rhizosphere bacteria is lowered than that in the wild sample,and the dominant taxa changed in the cultivated Morus mongolica rhizosphere bacteria,as well.The sequencing analysis based on the metagenomic DNA and 16S rRNA gene can reveal the composition of rhizosphere bacteria.
NETL
NSTL
万方数据
维普
