Analysis of Nitrogen Stress on DNA Methylation by MSAP in Tall Fescue
Tall fescue is an important herbage in gramineae, its growth and development process is directly influenced by the nitrogen concentration, although it has good cold resistance and heat resistance.DNA methylation plays a vital role in plant protection against adversity stress as a way of epigenetic regulation.We use tall fescue as test materials.Without nitrogen stress as control group, analysis of changes in genomic DNA methylation by methylation sensitive amplified polymorphism (MSAP), after 15 d no nitrogen stress.The results showed that: plant growth is strongly suppressed and large areas of yellow leaves, after 15 d nitrogen free treatment.Choose 12 pairs of primers for MSAP analysis, 725 gene loci were detected.The methylation and demethylation sites accounted for 16.4％.Total methylation level in the control group and nitrogen stress group were 65.56％ and 65.47％, respectively.Genomic DNA total methylation level did not change significantly in tall fescue under nitrogen stress in 15 days.Cloning and sequencing of article 15 marker bands, successfully get 14 different variation types of sequence.These sequences have high homology with gramineous plants, We hypothesized that tall fescue genomic methylation may occur in the region of the code under nitrogen stress.In conclusion, methylation changes under nitrogen stress induction, may participate in regulating environmental adaptability in tall fescue.
NETL
NSTL
万方数据
维普
