嗜水气单胞菌外膜囊泡组分及功能分析
Proteomics and Functional Analyses of Outer Membrane Vesicles in Aeromonas hydrophila
孙莉娜 1姚祖杰 1谭礼宁 1李碗芯 1胡文杰 1林玲 1林向民1
作者信息
- 1. 福建农林大学福建省农业生态过程与安全监控重点实验室,福州,350002;福建农林大学生命科学学院,福州,350002
- 折叠
摘要
嗜水气单胞菌是一种鱼类常见的致病菌,且每年养殖业因感染该菌所造成的损失不也计其数.据研究表明,细菌的外膜囊泡具有免疫保护功能,然而目前对嗜水气单胞菌OMVs组分及其功能的研究还鲜有报道.故本研究首次分离纯化得到嗜水气单胞菌分泌的OMVs,然后利用其免疫小鼠的血清进行抑菌实验,结果表明抗OMVs血清具有良好的抑菌效果.为了进一步了解嗜水气单胞菌的OMVs蛋白的特性,本研究通过利用蛋白质组学技术对其蛋白组分进行分析.经结果分析共鉴定到191个蛋白,包括外膜蛋白、脂蛋白、胞浆蛋白等;并涉及细菌氧化还原作用、跨膜转运与生物学合成等多种重要的生物学功能和过程.此外,还有一些如外膜蛋白OmpA等的毒力因子蛋白的发现,都为日后蛋白相关疫苗的研制提供了候选储备.通过本研究,我们更全面地了解嗜水气单胞菌外膜囊泡的生物学功能,不仅有助于筛选具有重要免疫保护性的蛋白,也为研制嗜水气单胞菌的相关疫苗奠定了研究基础.
Abstract
Aeromonas hydrophila is a common pathogen that infects a variety of fish and is widely found in water environment,leading to huge economic losses in aquaculture industry.Many studies showed that outer membrane vesicles,OMVs released from Gram-negative bacteria have an immune protective effect on the hosts.However,few studies about the component and function of A.hydrophila OMVs were reported.In this work,the purified OMVs were injected to mice and the immune serums were used to perform bactericidal assay.Results showed that the immune serum of OMVs can work as the bacteria inhibitor.To further understand the function ofA.hydrophila OMVs,we also investigated the proteomic composition and the immunostimulatory effect of OMVs released from A.hydrophila.According to the bioinformatics analysis,a total of 191 proteins was identified,including outer membrane proteins,lipoproteins and cytoplasmic proteins.These proteins are related to many important biological processes and molecular functions,including oxidoreductase activity,transmembrane transport activity and biosynthetic process.Moreover,some proteins work as the virulence factors delivered by A.hydrophila OMVs can be also played as the potential vaccine candidates in the future,such as out membrane protein,OmpA.An understanding of the biological functions of OMVs may be helpful for the development of strategies for screening immune protective proteins and would also provide a theoretical basis for the vaccine of A.hydrophila.
关键词
嗜水气单胞菌/外膜囊泡/蛋白质组学Key words
Aeromonas hydrophila/Outer membrane vesicles/Proteomics引用本文复制引用
基金项目
国家自然科学基金(31200105和31470238)
出版年
2015
NETL
NSTL
维普
万方数据