Identification and Optimization of Fermentation Conditions for Highly Efficient Feather Degrading Strains
The strain BS8 of highly effective degrading feather was obtained by the early research,which could be used to degrade the whole feathers in 48 h.The degradation ability of different animal hair was studied,and the effect of the strain on the feather keratin degradation was most obvious.The results showed that the strain was Bacillus subtilis by morphological,physiological and biochemical characteristics and 16S rDNA analysis.The optimum culture conditions of strain were optimized by single factor test and the orthogonal test was carried out.The experimental results showed that the optimum culture conditions for liquid fermentation of strain BS8:initial pH 8.0,fermentation temperature 37℃,inoculation amount of 2％,feather content of 1％,180 r/min shaking culture 48 h.Under these conditions,the keratinase activity of 23.6 U/mL was detected,which was increased by 29.7％ compared with the pre optimized of the 18.2 U/mL.The production of waste feather by strain BS8 was not only beneficial to environmental protection,but also might be widely used in feed protein source and organic fertilizer.
NETL
NSTL
万方数据
维普
