Screen of Grifola frondosa Thermotolerant Strains and Analysis of the Genetic Relationship of Candidate Strains
We screened two Grilfola frondosa strians Gr0001 +3 and Gr0017,which can fruit normally under 21.5～23.5℃,from forty candidate Grilfola frondosastrains.We observed that the fruitbody characters of Gr0001 +3 were better than that of Gr0017,so Grilfola frondosastrain Gr0001 +3 would be chosen as a parent for breeding offsprings that are tolerent with high temperature.In our study,we employed RAPD and ISSR to analyze the genetic diversity of the forty Grilfola frondosa candidate strains.Eight RAPD primers resulted in eighty-three specific bands,and divided the forty Grilfola frondosa strains into nine groups when the dissimilarity coefficient is 0.455.Nine ISSR primers resulted in sixty-three specific bands,and divided the forty candidate Grilfola frondosa strains into five groups when the dissimilarity coefficient is 0.63.The comprehensive analysis result showed that the clustering figure indicated the genetic relationship of the forty strains at molecular level.From the corresponding analysis,we can see that the two strains that could fruit at 21.5～23.5 ℃ have a close genetic relationship,which will supply theoretical foundation for parent choice in Grilfola frondosa genetic breeding.
NETL
NSTL
万方数据
维普
