In order to evaluate the genetic diversity and relationship of Guangxi Longlin pigs,Debao pigs and Shitou pigs,17 microsatellite markers microsatellite were analyzed.The genetic structural variation of the 3 pigs was analyzed by calculating their microsatellite genetic diversity parameters.The results showed that 199 alleles were detected in 61 individuals of Longlin pigs,Debao pigs and Shitou pigs and the number of each locus was 8~19;The average polymorphic information content was 0.717 5 and expected heterozygosity were 0.715 2,0.800 9,0.794 2,indicating they are rich in polymorphic information and display a wide range of genetic variability;Genetic distances were 0.461 6,0.322 6,0.278 2 and genetic similarity coefficient were 0.630 3,0.724 3,0.757 2.Results revealed that there were some gene flow among the 3 pigs,roviding a theoretical basis rational use and protection for the germplasm resources of Guangxi local swine.