Analysis of the Young Leaf Transcriptome of Highbush Blueberry Variety "Ruilan"
Blueberry was known as "the king of small berries" for rich in nutrition and antioxidant substances such as anthocyanin.In order to explore the molecular mechanism of blueberry development and growth,in this study,the leaf transcriptome of 2 year old blueberry variety ‘Ruilan’ was sequenced by the second generation sequencing technology.The bioinformatics analysis showed that:total about 1 G clean reads could been assembled into 41 987 unigenes.Among them,23 555 unigenes could been annotated by dataset GO,which are involving 35 kinds metabolism and physiological function of biological process,cellular component and molecular function;20 379 unigenes could been annotated by KOG dataset,which are involving in 25 metabolism pathway;9 771 unigenes cauld been annotated by KEGG dataset,which are involving in five major categories,20 middle categories,and 124 metabolism pathways;27 293 unigenes could been annotated by dataset Nr,which are mapping into 32 949 code regions of protein.We also find 5 221 unigenes been the candidates gene sequences of transcript factor of 54 families,and 2 487 unigenes which code the R gene of 19 families;A total of 6 261 SSR sequences were detected,of which 2 base repeat SSR accounted for 68.2％.The results of the study is great helpful to understand the growth and development molecular mechanism of"Ruilan",has important significance to use this germplasm for blueberry breeding and improvement.
NETL
NSTL
万方数据
维普
