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羚牛线粒体基因组全序列测定与分析

Analysis on Complete Mitochondrial Genome Sequence of Golden Takin (Budorcas taxicolor)

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利用长距离PCR扩增法对秦岭羚牛线粒体基因组进行测序、拼接、注释并对基因组特点和羚牛系统发育进行分析.结果表明,羚牛线粒体基因组全长为16 662 bp,GenBank序列号:KU361169.该基因由13个蛋白编码基因、22个转运RNA基因、2个核糖体RNA基因和1个非编码控制区组成.基因组核苷酸组成为A:33.9%,T:27.0%,C:26.3%,G:12.8%,(A+T)含量(60.9%)高于(G+C)含量(39.1%),有一定的碱基偏好.ND2、ND3、ND5基因起始密码子为ATA,其余10个蛋白质编码基因起始密码子为ATG.ND2、ND3、ND4、COⅢ基因为不完全终止密码子T,其余蛋白质基因为完全终止密码子TAA和AGA.预测了22个tRNA二级结构,共有38处错配,以GU错配为主.基于14种有蹄类动物线粒体基因组构建的系统发生树,表明羚牛与山羊亲缘关系最近,应归为羊亚科.
The complete mitochondrial genome sequence of Budorcas taxicolor was determined,spliced and annotated by long PCR.The phylogenetic relationship ofBudorcas taxicolor was analyzed.The results showed that the total length of mitochondrial genome of Budorcas taxicolor was 16 662 bp (the accession number of GenBank:KU361169).The mitogenome contained 13 protein coding genes,22 tRNA,2 rRNA,and a control region.The nucleotide composition was A:33.9%,T:27.0%,C:26.3%,G:12.8%,(A+T) (60.9%) was higher than (G+C) (39.1%).There was a strong A-T bias.The mitochondrial protein coding genes ND2,ND3 and ND5 used ATA as initiation codon,other 10 protein coding genes used ATG.The ND2,ND3,ND4 and CO Ⅲ genes had in-complete termination codon (T),while others used termination codons (TAA and AGA) as usual.The secondary structures of 22 tRNAs were predicted.There were 38 mismatch most of which were GU mismatch.The BI phylogenetic tree was built by the complete mitochondrial genome sequences of 14 Ungulata animals.The relationship ofBudorcas taxicolor and Capra aegagrus hircus was closest.The result supported Budorcas taxicolor should be placed into Caprinae.

Budorcas taxicolorComplete mitochondrial genomeRNA secondary structurePhylogenetic relationship

冯慧、冯成利、黄原、王璐

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陕西省动物研究所,西安,710032

陕西师范大学,西安,710062

羚牛 线粒体基因组 RNA二级结构 系统进化

陕西省13115重大科技专项陕西省科技计划项目陕西省科学院青年人才培养项目

2010ZDKG-542012KW-212009K-02

2016

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCDCSCD北大核心
影响因子:1.108
ISSN:1674-568X
年,卷(期):2016.35(9)
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